Coding

Part:BBa_K2612001:Design

Designed by: Elisha Krauss   Group: iGEM18_Queens_Canada   (2018-10-10)


4-HT Intein in Kanamycin Resistance


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 690


Design Notes

The kanamycin resistance gene was split at Amino Acid 119. The 119th amino acid was also removed, because intein splicing results in a cysteine scar.


Source

This part was first constructed and detailed in this papaer: Buskirk AR, Ong Y-C, Gartner ZJ, Liu DR. Directed evolution of ligand dependence: Small-molecule-activated protein splicing. Proceedings of the National Academy of Sciences of the United States of America. 2004;101(29):10505-10510. doi:10.1073/pnas.0402762101.

References